Stabilize Blood Samples for Convenient Sample Collection and Transportation
Ardent Cell-Free DNA Blood Collection Tube is used for isolation of high quality cell-free DNA from plasma. The collection tube contains unique anticoagulants and preservatives, which prevent cell-free DNA from degradation, stabilizes the white blood cells and minimizes the release of genomic DNA from nucleated blood cells without inference of PCR amplification of extracted cell-free DNA.
Samples collected in Ardent Cell-Free DNA Blood Collection Tubes are stable for up to 7 days at room temperature, which allows convenient sample collection, transport, and storage.
Produce High Yield of Plasma Cell-Free DNA and Has No PCR Inhibition
Samples collected in Ardent Cell-Free DNA Blood Collection Tubes can give high yield of plasma cell-free DNA using magnetic beads and membrane based DNA extraction, the common DNA extraction methods. There is no PCR inhibition of extracted DNA for downstream molecular analysis. Figure 1 demonstrates the higher cell-free DNA yield and better PCR amplification from Ardent Cell-Free DNA Blood Collection Tubes than the similar products in the market.
Figure 1. Better DNA Yield， No Inhibition of PCR Amplification and High Quality of DNA Library



Be Easy to Use and Reduce Errorsbr Ardent Cell-Free DNA Blood Collection Tubes have unique design of easy removable dual barcodes on tube and blood collection volume mark labeling. The easy peeling-off tube barcode is convenient and avoids tube labeling errors during sample plasma separation. The volume marks guide the collection of correct blood volume.
For Research Use Only. Not for Use in Diagnostic Procedures.

Application
The cellular and molecular analysis of circulating rare such as circulating tumor cells (CTC), circulating fetal cells (CFC) and circulating stem cells (CSC), usually requires extensive enrichment procedures. Those cells are very fragile and get lost/lysed during enrichment processes.
Ardent Blood Cell Collection Tubes containing reagents that both stabilize fragile cell membranes of circulating rare cells without interfering downstream molecular and cellular analyses and strong anti-blood clotting and anti-platelet-aggregation, are extremely useful in collecting blood samples for the analyses of circulating rare blood cells, which may include
Circulating tumor cells (CTC)
Circulating fetal cells (CFC)
Circulating stem cells (CSC)
Circulating endothelial cells (CEC)
Circulating micro-organism (bacteria, viruses and fungus, etc.)
Subpopulation of white blood cells
Microfluidics and Flow Cytometers have been frequently used in the process.
For Research Use Only. Not for Use in Diagnostic Procedures.

Ardent Blood Cell Collection Tube
Stabilize Whole Blood Cells for Convenient Blood Sample Collection and Transportation Blood samples collected in regular anticoagulants usually should be processed within 6 hours after blood drawing for cell analysis, since the membranes of white blood cells normally become unstable and start releasing various protein enzymes 13 hours post-drawing, degrading the whole blood samples.
Ardent Blood Cell Collection Tubes contain proprietary anticoagulants and preservatives, which not only stabilize whole blood cells, but also effectively prevent blood clotting and platelet aggregation. The blood samples collected in Ardent Blood Cell Collection Tubes can be transported and stored at room temperature for 36 to 72 hours for downstream cellular and molecular analysis.
Provide Excellent Blood Cell Stabilization and Anti-Blood Clotting without Interfering Downstream Cellular and Molecular Analysis
Cross-linking preservatives like formaldehyde have good cell preservation, but may interact with cell-surface proteins and receptors affecting cell analysis, and inhibit the PCR amplification of extracted DNA and RNA. Ardent Blood Cell Collection Tubes contain unique reagents that can effectively stabilize cell membranes and prevent blood clotting without significantly affecting downstream cellular and molecular analysis. Figure